Zinc Aptamer Details
ID# 7550
Structure
Chemistry
RNA
Target
Zinc
Category
Small Organic
Affinity (Kd)
1.2 mM
Length
95
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprGprAprGprAprGprGprAprUprAprCprUprAprCprUprGprUprCprAprUprAprCprGprUprUprAprGprGprCprUprGprUprAprGprGprCprGprAprGprGprUprGprAprAprAprUprGprAprGprCprGprGprUprAprAprUprAprGprCprCprUprCprAprGprCprGprUprAprGprCprAprUprAprUprGprCprAprUprGprAprAprUprUprCprGprAprAprGprCprUprUprCprGprCp-3'
Molecular Weight: 30777.6
Extinction Coefficient: 966700
Binding Conditions / Buffer
Buffer A (0.4 M NaCl, 20 mM HEPES-Na (pH 7.0), and 1 mM MgCl2)
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Ciesiolka et al., "Selection of an RNA domain that binds Zn2+." Journal of RNA, 1(1995): 538-550.
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