Yeast phenylalanine tRNA (B2) Aptamer Details
ID# 7459
Structure
Chemistry
RNA
Target
Yeast phenylalanine tRNA
Category
Small Organic
Affinity (Kd)
12 nM
Length
109
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprGprAprAprUprUprCprCprGprCprGprUprGprUprGprCprUprAprCprGprUprAprUprCprUprUprCprAprGprGprCprGprGprUprAprAprCprUprAprAprCprUprGprUprGprCprUprGprAprGprUprCprUprAprAprUprCprUprUprUprGprUprGprAprGprGprGprAprCprGprGprUprAprAprCprAprUprAprUprGprGprUprUprCprCprCprGprCprGprUprGprGprUprCprCprGprUprUprCprGprGprGprAprUprCprCprUprCp-3'
Molecular Weight: 35011.9
Extinction Coefficient: 1060400
Binding Conditions / Buffer
Selection Buffer (250 mM NaCl, 50 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 2 mM spermidine, 0.2 mM EDTA)
Binding Temp: 25°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Scarabino, Daniela, et al. "tRNA prefers to kiss." EMBO Journal 18 (1999): 4571-4578
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