WT62 Aptamer Details
ID# 8071
Structure
Chemistry
DNA
Target
HIV-1 Reverse Transcriptase
Category
Protein
Affinity (Kd)
7.51±0.29x10^-8 nM
Length
41
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5’-dTpdCpdGpdGpdTpdTpdGpdApdGpdTpdGpdApdGpdApdTpdTpdApdGpdTpdCpdApdApdApdTpdGpdTpdTpdTpdApdGpdApdApdGpdApdCpdApdApdCpdCpdTpdCp-3’
Molecular Weight: 12687.3
Extinction Coefficient: 412100
Binding Conditions / Buffer
10 mM HEPES pH 7.5, 150 mM NaCl, 80 mM KCl, and
5 mM MgCl2
Binding Temp: 25°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Hannongbua, S., Ratanabunyong, S., Aeksiri, N., Yanaka, S., Yagi-Utsumi, M., Kato, K., & Choowongkomol, K. (2020). Characterization of new DNA Aptamers for anti‐HIV‐1 Reverse Transcriptase. ChemBioChem. doi:10.1002/cbic.202000633
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