VRF-HS_B7H3-01 (Aptamer Against B7H3, Retinoblastoma, Weri-RB1) Aptamer Details
ID# 8803
Structure
Chemistry
DNA
Target
B7H3
Category
Protein
Affinity (Kd)
32.12 nM
Length
86
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
dTpdApdGpdGpdGpdApdApdGpdApdGpdApdApdGpdGpdApdCpdApdTpdApdTpdGpdApdTpdCpdGpdCpdCpdCpdCpdTpdCpdTpdTpdTpdCpdGpdApdTpdTpdTpdCpdApdApdGpdCpdTpdCpdCpdTpdApdApdCpdCpdCpdApdCpdApdCpdCpdTpdCpdTpdTpdTpdGpdApdCpdTpdApdGpdTpdApdCpdApdTpdGpdApdCpdCpdApdCpdTpdTp
Molecular Weight: 26668.3
Extinction Coefficient: 857400
Binding Conditions / Buffer
25 mM Glucose, 0.9 mM CaCl2, 5 mM MgCl2 in Dulbecco’s PBS without calcium and magnesium, 0.1 mg/ml yeast tRNA, 0.1 mg/ml Salmon sperm DNA, 5% FBS, and 1 mg/ml BSA.
Binding Temp: 4°C
Refolding Protocol
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Maradani, B.S., Parameswaran, S. & Subramanian, K. Development of DNA aptamers targeting B7H3 by hybrid-SELEX: an alternative to antibodies for immuno-assays. Sci Rep 14, 13552 (2024). https://doi.org/10.1038/s41598-024-64559-7
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