VRF-CSRB-01 Aptamer Details

ID# 8234

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Structure

Chemistry

DNA

Target

Retinoblastoma (RB) (Weri-RB1 cells)

Sequence (3-char table code)

G A C U/T

5'-dTpdApdGpdGpdGpdApdApdGpdApdGpdApdApdGpdGpdApdCpdApdTpdApdTpdGpdApdTpdTpdCpdApdApdApdGpdTpdApdApdCpdTpdCpdTpdGpdTpdCpdApdCpdApdGpdTpdApdCpdApdApdTpdApdGpdApdTpdTpdCpdTpdCpdApdTpdTpdApdTpdCpdApdTpdTpdGpdApdCpdTpdApdGpdTpdApdCpdApdTpdGpdApdCpdCpdApdCpdTpdTpdGpdAp-3'

Molecular Weight:

Extinction Coefficient:

Binding Conditions / Buffer

Prior to each round, the native ssDNA library dissolved in Binding Buffer [BB–Wash Buffer with 0.1 mg/mL yeast tRNA (Invitrogen), 0.1 mg/mL Salmon sperm DNA (Ambion, Life technologies), 5% FBS (Gibco) and 1 mg/ml BSA (Sigma)]

Binding Temp: 20°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Maradani, B.S., Parameswaran, S. & Subramanian, K. Development and characterization of DNA aptamer against Retinoblastoma by Cell-SELEX. Sci Rep 12, 16178 (2022). https://doi.org/10.1038/s41598-022-20660-3 https://www.nature.com/articles/s41598-022-20660-3#citeas

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