von Willebrand Factor (VWF R9.3) Aptamer Details
ID# 7579
Structure
Chemistry
2'-F-RNA
Target
von Willebrand Factor (VWF)
Category
Protein
Affinity (Kd)
1.2 nM
Length
76
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprGprAprGprGprApfCprGprApfUprGpfCprGprGp rApfUpfCp rGpfCprGpfCp fUpfCpfUp fCpfCpfUp rGpfCpfUp fUprAprAp rGpfCprAp rGpfCpfUp rApfUpfCp rAprAprAp fUprAprGp fCpfCpfCp rApfCpfUp fCprAprGprApfCprGprApfCpfUpfCprGpfCpfUprGprAprGprGprApfUpfCpfCp -3'
Molecular Weight: 24492.61
Extinction Coefficient: 731800
Binding Conditions / Buffer
For nitrocellulose filter binding assays:
Selection Buffer E (20mM HEPES (pH 7.4), 50 mM NaCl, 2 mM CaCl2, and 0.1% BSA)
-OR-
Selection Buffer F (20mM HEPES (pH 7.4), 150 mM NaCl, 2 mM CaCl2, and 0.1% BSA)
For platelet function analysis(performed at RT):
Platelet binding buffer: 150 mM NaCl, 20 mM Hepes pH 7.5; 5 mM KCL; 1 mM MgCl2, and 1 M CaCl2.
Binding Temp: 37°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
S Oney, BA Sullenger, et al. (2007) "Antidote-Controlled Platelet Inhibition Targeting von Willebrand Factor with Aptamers." Oligonucleotides, 17:265-274.
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