von Willebrand Factor (VWF R9.14) Aptamer Details

ID# 7846

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Structure

Chemistry

2'-F-RNA

Target

von Willebrand Factor (VWF)

Sequence (3-char table code)

G A C U/T

5'-rGprGprGprAprGprGprApfCprGprApfUprGpfCprGprGp fUprGprGp rApfCprGp rAprApfCp fUprGpfCp fCpfCpfUp fCprAprGp fCpfUprAp fCpfUpfUp fUpfCprAp fUprGpfUp fUprGpfCp fUprGprAp fCprGpfCp rAp fCprAprGprApfCprGprApfCpfUpfCprGpfCpfUprGprAprGprGprApfUpfCpfCp -3'

Molecular Weight: 24563.62

Extinction Coefficient: 731400

Binding Conditions / Buffer

For nitrocellulose filter binding assays: Selection Buffer E (20mM HEPES (pH 7.4), 50 mM NaCl, 2 mM CaCl2, and 0.1% BSA) -OR- Selection Buffer F (20mM HEPES (pH 7.4), 150 mM NaCl, 2 mM CaCl2, and 0.1% BSA) For platelet function analysis(performed at RT): Platelet binding buffer: 150 mM NaCl, 20 mM Hepes pH 7.5; 5 mM KCL; 1 mM MgCl2, and 1 M CaCl2.

Binding Temp: 37°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

S Oney, BA Sullenger, et al. (2007) "Antidote-Controlled Platelet Inhibition Targeting von Willebrand Factor with Aptamers." Oligonucleotides, 17:265-274.

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