VEGF (SL2-B aptamer) Aptamer Details
ID# 7688
Structure
Chemistry
DNA
Target
VEGF (165)
Category
Protein
Affinity (Kd)
0.5 nM
Length
26
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dCpdApdApdTpdTpdGpdGpdGpdCpdCpdCpdGpdTpdCpdCpdGpdTpdApdTpdGpdGpdTpdGpdGpdGpdTp-3'
Molecular Weight: 8034.25
Extinction Coefficient: 242600
Binding Conditions / Buffer
PBS and 0.005% tween-20 solution mixture was used as the running buffer, and 50 mM NaOH as the regeneration buffer.
Binding Temp: 25°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Kaur H, Yung L-YL (2012) Probing High Affinity Sequences of DNA Aptamer against VEGF165. PLoS ONE 7(2): e31196. doi:10.1371/journal.pone.0031196
Request a Quote
Have your aptamer oligo synthesized by Aptagen.
