VEGF receptor Flt-1 (F56) Aptamer Details
ID# 7630
Structure
Chemistry
Peptide
Target
VEGF receptor Flt-1
Category
Cells
Affinity (Kd)
N/A nM
Length
12
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
NH2-TrpHisSerAspMetGluTrpTrpTyrLeuLeuGly-COOH
Molecular Weight: 1759.02
Extinction Coefficient:
Binding Conditions / Buffer
Washing Buffer (150 mM NaCl and 10 mM phosphate buffer, pH 6.2) and then blocked in 3% BSA (25 mM HEPES, pH 7.6) for 3 hours at room temperature.
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
An et al. "Suppression of Tumor Growth and Metastasis by a VEGFR-1 Antagonizing Peptide Identified from a Phage Display Library." Int. J. Cancer, 111(2004): 165ƒ??173.
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