Vaspin binding aptamer (VBA) Aptamer Details
ID# 7774
Structure
Chemistry
DNA
Target
Vaspin
Category
Protein
Affinity (Kd)
303 nM
Length
75
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dApdTpdAp dCpdCpdAp dGpdCpdTp dTpdApdTp dTpdCpdAp dApdTpdTp dGpdGpdGp dCpdGpdGp dTpdGpdGp dGpdGpdGp dGpdGpdGp dTpdApdGp dTpdGpdGp dGpdTpdGp dTpdTpdAp dTpdGpdGp dCpdGpdAp dTpdCpdGp dTpdGpdGp dApdGpdAp dTpdApdGp dTpdApdAp dGpdTpdGp dCpdApdAp dTpdCpdTp-3'
Molecular Weight: 23487.31
Extinction Coefficient: 743100
Binding Conditions / Buffer
Binding Buffer: 100 mM NaCl, 25 mM Tris-HCl, 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, and 0.02% Tween-20, pH 7.6.
Binding Temp: RT°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
SJ Lee et al. Sensitive detection of adipokines for early diagnosis of type 2 diabetes using enzyme-linked antibody-aptamer sandwich (ELAAS) assays. Sensors Actuat. B-Chem.(2012): 243-248.
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