Vascular Permeability Factor/Vascular Endothelial Growth Factor (NX-213) Aptamer Details
ID# 7504
Structure
Chemistry
RNA
Target
Nickel
Category
Small Organic
Affinity (Kd)
800 nM
Length
89
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprGprAprGprAprGprGprAprUprAprCprUprAprCprAprCprGprUprGprGprAprAprAprAprAprCprCprAprAprCprAprAprAprUprUprGprGprGprAprAprAprAprAprUprGprUprUprAprAprGprGprGprUprCprCprAprCprUprUprCprAprUprGprCprCprAprUprUprGprCprAprUprGprUprAprGprCprAprGprAprAprGprCprUprUprCprCprGp-3'
Molecular Weight: 28782.5
Extinction Coefficient: 912100
Binding Conditions / Buffer
Ni-NTA resin (200 uL) equilibrated with a buffer (50 mM NaCl, 7 mM MgCl2, 50 mM Tris-HCl (pH 7.5) was heated at 75C for 3 min and then slowly cool to RT. The Sample was loaded on the Ni-NTA matrix for 5 min under gentle agitation.
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Hoffman, H., et al. "Ni2+-binding RNA motifs with an asymmetric purine-rich internal loop and a G-A base pair." RNA Journal, 3 (1997): 1289-1300.
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