Vaccinia virusƒ??infected A549 cells (TV08) Aptamer Details
ID# 7663
Structure
Chemistry
DNA
Target
Vaccinia virus (WR Strain)ƒ??infected A549 cells
Category
Cells
Affinity (Kd)
2.7 nM
Length
83
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dApdTpdCpdGpdTpdCpdTpdGpdCpdTpdCpdCpdGpdTpdCpdCpdApdApdTpdApdTpdGpdApdTpdGpdApdCpdApdCpdCpdTpdGpdCpdApdTpdApdApdTpdTpdTpdApdTpdApdGpdTpdGpdApdGpdTpdCpdTpdTpdGpdApdTpdTpdCpdApdCpdGpdCpdTpdGpdCpdApdTpdTpdTpdGpdGpdTpdGpdTpdGpdApdGpdGpdTpdCpdGpdTpdGpdCp-3'
Molecular Weight: 25569.65
Extinction Coefficient: 784600
Binding Conditions / Buffer
Binding Buffer: Dulbecco PBS, 4.5g/L glucose, 5 mmol/L MgCl2, 0.1 g/L yeast tRNA, 100 mL/L FBS, and 1 g/L BSA.
Binding Temp: 4°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Tang et al. "Generating Aptamers for Recognition of Virus-Infected Cells." Clin. Chem., 55(2009): 813-822.
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