V7t1 targeting VEGF-165 Aptamer Details

ID# 7734

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Structure

Chemistry

DNA

Target

VEGF-165

Sequence (3-char table code)

G A C U/T

5'-dTpdGpdTpdGpdGpdGpdGpdGpdTpdGpdGpdApdCpdGpdGpdGpdCpdCpdGpdGpdGpdTpdApdGpdAp-3'

Molecular Weight: 7900.16

Extinction Coefficient: 248000

Binding Conditions / Buffer

10% (v/v) human serum in TBSTE (TBSE containing 0.05% (v/v) of Tween-20).

Binding Temp: 24°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Nonaka,Y., Sode, K., and Ikebukuro, K.(2010) "Screening and Improvement of an Anti-VEGF DNA Aptamer." Molecules, 15, 215-225. Stejskalov?, Anna & Oliva, Nuria & J England, Frances & Almquist, Benjamin. (2019). Biologically Inspired, Cell-Selective Release of Aptamer-Trapped Growth Factors by Traction Forces. Advanced Materials. 1806380. 10.1002/adma.201806380.

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