V7t1 targeting VEGF-121 Aptamer Details
ID# 7733
Structure
Chemistry
DNA
Target
VEGF-121
Category
Protein
Affinity (Kd)
1.1 nM
Length
25
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dTpdGpdTpdGpdGpdGpdGpdGpdTpdGpdGpdApdCpdGpdGpdGpdCpdCpdGpdGpdGpdTpdApdGpdAp-3'
Molecular Weight: 7900.16
Extinction Coefficient: 248000
Binding Conditions / Buffer
10% (v/v) human serum in TBSTE (TBSE containing 0.05% (v/v) of Tween-20).
Binding Temp: 24°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Nonaka,Y., Sode, K., and Ikebukuro, K.(2010) "Screening and Improvement of an Anti-VEGF DNA Aptamer." Molecules, 15, 215-225.
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