V. parahemolyticus (A3P) Aptamer Details
ID# 7671
Structure
Chemistry
DNA
Target
Vibrio parahemolyticus
Category
Cells
Affinity (Kd)
16.88 nM
Length
87
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dApdTpdApdGpdGpdApdGpdTpdCpdApdCpdGpdApdCpdGpdApdCpdCpdApdGpdApdApdTpdCpdTpdApdApdApdApdApdTpdGpdGpdGpdCpdApdApdApdGpdApdApdApdCpdApdGpdTpdGpdApdCpdTpdCpdGpdTpdTpdGpdApdGpdApdTpdApdCpdTpdTpdApdTpdGpdTpdGpdCpdGpdTpdCpdTpdApdCpdCpdTpdCpdTpdTpdGpdApdCpdTpdApdApdTp-3'
Molecular Weight: 26893.59
Extinction Coefficient: 870500
Binding Conditions / Buffer
Binding Buffer: 50 mM Tris-HCl (pH 7.4), 5 mM KCl, 100 mM NaCl, and 1 mM MgCl2
Binding Temp: RT°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Duan, N et al. Selection and identification of a DNA aptamer targeted to Vibrio parahemolyticus. J. Agri. Food Chem., 60(2012): 4034-4038.
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