Uranium DNAzyme Aptamer Details
ID# 7950
Structure
Chemistry
DNA
Target
Uranium
Category
Other
Affinity (Kd)
n/a nM
Length
54
Aptazyme
Yes
Sequence (3-char table code)
G
A
C
U/T
dCpdApdCpdGpdTp dCpdCpdApdTpdCp dTpdCpdTpdGpdCp dApdGpdTpdCpdGp dGpdGpdTpdApdGpdTpdTpdApdApdAp dCpdCpdGpdApdCp dCpdTpdTpdCpdAp dGpdApdCpdApdTp dApdGpdTpdApdAp dGpdGpdGpdGp
Molecular Weight: 16630.84
Extinction Coefficient: 524500
Binding Conditions / Buffer
0.4 M NaCl and 20 mM MES buffer solution (pH 5.5) at 20?C for 15 min. To quench: 5 uL of 0.5 M Tris base solution added then sample was vortexed to shift the pH from 5.5 to 8.0.
Binding Temp: 20°C
Refolding Protocol
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Sai Jin Xiao, Jun Zuo, Zhi Qiang Zhu, Yong Zhong Ouyang, Xing Lei Zhang, Huan Wen Chen, & Li Zhang (2015) Highly Sensitive DNAzyme Sensor for Selective Detection of Trace Uranium in Ore and Natural Water Samples. Sensors and Actuators B 210 (2015) 656-660
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