Unphosphorylated ERK2 Aptamer Details
ID# 7655
Structure
Chemistry
RNA
Target
Unphosphorylated ERK2
Category
Protein
Affinity (Kd)
2 nM
Length
75
Aptazyme
Yes
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprCp rGprUprGp rAprCprCp rUprGprAp rUprGprAp rGprUprCp rAprCprGp rCprUprAp rAprGprGp rAprGprGp rAprUprUp rUprCprCp rGprAprAp rAprGprCp rGprGprCp rUprAprCp rGprGprUp rCprCprGp rCprCprAp rGprUprGp rUprUprAp rCprGprAp rAprAprCp rGprUprUp rCprCprCp -3'
Molecular Weight: 24170.59
Extinction Coefficient: 732700
Binding Conditions / Buffer
34-37 °C, 10 mM Tris-HCl, pH 7.5, 10 mM MgCl2, 0.05 μg/μl tRNA
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Vaish et al. "Monitoring post-translational modifications of proteins with allosteric ribozymes." Nature Biotechnology, 20(2002): 810-815.
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