UA-Mg-1 Aptamer Details
ID# 8237
Structure
Chemistry
DNA
Target
Uric acid (UA)
Category
Other
Affinity (Kd)
7-8 nM
Length
73
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdGpdApdGpdGpdCpdTpdCpdTpdCpdGpdGpdGpdApdCpdGpdApdCpdGpdGpdGpdCpdApdApdGpdApdGpdGpdTpdTpdTpdTpdApdCpdTpdTpdApdCpdCpdTpdApdApdGpdGpdApdApdTpdGpdTpdCpdGpdTpdGpdTpdCpdGpdTpdCpdCpdCpdGpdApdCpdTpdCpdTpdApdTpdGpdApdTpdGpdApdCpdTpdGpdTp-3'
Molecular Weight:
Extinction Coefficient:
Binding Conditions / Buffer
Alternating Na+ and Li+ in the selection rounds, and both buffers contained 10 mMMg2+. In the end, a Mg2+-dependent aptamer was isolated to bind to UA.
The Na+ buffer contained 150 mM NaCl, while the Li+buffer contained 150 mM LiCl. Both buffers contained 20 mM Tris ,pH 8.3, 10 mM MgCl2and 10 mM KCl.
Binding Temp: 80°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Liu, Y., Liu, J., ChemBioChem 2023, 24, e202200564.
https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/cbic.202200564
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