TV06 Aptamer Details
ID# 8067
Structure
Chemistry
DNA
Target
STIP1 (TOV-21G, Ovarian Cancer)
Category
Protein
Affinity (Kd)
4.5 g/L glucose and 5 mM MgCl2 in Dulbecco’s phosphate-buffered saline (PBS) with CaCl2 and MgCl2 (Sigma), baker’s yeast tRNA
Length
84
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dApdTpdCp dCpdApdGp dApdGpdTp dGpdApdCp dGpdCpdAp dGpdCpdAp dCpdGpdGp dCpdApdCp dTpdCpdAp dCpdTpdCp dTpdTpdTp dGpdTpdTp dApdApdGp dTpdGpdGp dTpdCpdTp dGpdCpdTp dTpdCpdTp dTpdApdAp dCpdCpdTp dTpdCpdAp dTpdCpdGp dApdCpdAp dCpdGpdGp dTpdGpdGp dCpdTpdTp dAp-3'
Molecular Weight: 23311.1
Extinction Coefficient: 708300
Binding Conditions / Buffer
4°C
Binding Temp: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Van Simaeys, D., Turek, D., Champanhac, C., Vaizer, J., Sefah, K., Zhen, J., … Tan, W. (2014). Identification of Cell Membrane Protein Stress-Induced Phosphoprotein 1 as a Potential Ovarian Cancer Biomarker Using Aptamers Selected by Cell Systematic Evolution of Ligands by Exponential Enrichment. Analytical Chemistry, 86(9), 4521–4527. doi:10.1021/ac500466x
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