Tumor necrosis factor-alpha (TNFa) (VR11) Aptamer Details
ID# 7741
Structure
Chemistry
DNA
Target
Tumor necrosis factor-alpha (TNFa)
Category
Protein
Affinity (Kd)
7 nM
Length
25
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'- dTpdGpdGpdTpdGpdGpdApdTpdGpdGp dCpdGpdCpdApdGpdTpdCpdGpdGpdCp dGpdApdCpdApdAp-3'
Molecular Weight: 7788.1
Extinction Coefficient: 244400
Binding Conditions / Buffer
Selection Buffer: PBS, 0.005% (v/v) Tween-20.
Binding Temp: 37°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
E Orava et al. A short DNA aptamer that recognizes TNFa and blocks its activity in vitro. ACS Chem. Biol. (2012) Accepted for print. DOI: 10.1021/cb3003557.
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