Tumor-derived endothelial cells (PepMW3) Aptamer Details

ID# 7748

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Structure

Chemistry

Peptide

Target

Tumor-derived endothelial cells (TDECs)

Sequence (3-char table code)

G A C U/T

5'-CysGlyGlyArgArgLeuGlyGlyCys-3'

Molecular Weight: 960.21

Extinction Coefficient:

Binding Conditions / Buffer

IMC Medium: 1X M9 Salts (40 mM Na2HPO4, 20 mM KH2PO4, 8.5 mM NaCl, 20 mM NH4Cl, pH 7.4), 0.2% casamino acids, 0.5% glucose, 1 mM MgCl2. Incubation/Wash solution: IMC medium containing 10% fetal calf serum (FCS) and 1% a-methyl mannoside.

Binding Temp: 4°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

C K Brown et al. A novel approach for the identificaiton of unique tumor vasculature binding peptides using an E. coli peptide display library. Ann Surg Oncol. 7(2000): 743-749.

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