TTF1 (A) Aptamer Details

ID# 7468

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Structure

Chemistry

DNA

Target

Thyroid Transcription Factor 1

Sequence (3-char table code)

G A C U/T

5'-dGpdGpdTpdApdTpdTpdGpdApdGpdGpdGpdTpdCpdGpdCpdApdTpdCpdTpdCpdApdApdApdApdGpdGpdGpdGpdTpdGpdApdTpdTpdGpdCpdTpdTpdGpdCpdApdCpdApdApdTpdGpdApdCpdApdGpdGpdGpdTpdApdGpdGpdApdCpdApdGpdApdTpdGpdGpdCpdTpdCpdTpdApdApdCpdTpdCpdTpdCpdCpdTpdCpdTp-3'

Molecular Weight: 24171.74

Extinction Coefficient: 753300

Binding Conditions / Buffer

Ni-NTA magnetic bead (Qiagen) were used with PBS-T (50 mM K2HPO4 pH 7.5, 150 mM NaCl, 0.05% Tween-20)

Binding Temp: 4°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Murphy et al. "An improved method for the in vitro evolution of aptamers and applications in protein detection and purification." Nucleic Acids Research, 31 (2003): e110.

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