TTA1.2 Aptamer Details
ID# 8052
Structure
Chemistry
2'-F-RNA
Target
Tenascin-C (TN-C)
Category
Protein
Affinity (Kd)
2.4 nM
Length
38
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-bGnbGnbGnbAnbGnmGpmApfCprGpfCprGpfUpfCprGpfCpfCprGpfUpmApmApfUpmGpmGpmApfUpmGpfUpfUpfUpfUpmGpbCnbUnbCnbCnbCnfUpmGp-3'
Molecular Weight:
Extinction Coefficient:
Binding Conditions / Buffer
TBSMC buffer (20 mM Tris, pH 7.4; 137 mM NaCl; 1 mM CaCl2; 1 mM MgCl2)
Binding Temp: 37°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Schmidt KS, Borkowski S, Kurreck J, Stephens AW,
Bald R, Hecht M, et al. Application of locked nucleic
acids to improve aptamer in vivo stability and targeting function. Nucleic Acids Res 2004; 32:5757-
65; PMID:15509871; http://dx.doi.org/10.1093/nar/
gkh862
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