Trypanosoma brucei (2-16) Aptamer Details
ID# 7548
Structure
Chemistry
RNA
Target
Trypanosoma brucei
Category
Cells
Affinity (Kd)
0.75 nM
Length
85
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprGprAprGprAprCprGprAprUprAprUprUprCprGprUprCprCprAprUprUprCprGprGprGprUprGprGprCprCprCprGprUprGprUprCprUprGprAprGprCprGprGprGprGprAprCprGprGprCprCprAprCprUprUprGprAprGprCprGprCprCprGprCprUprGprUprCprCprGprAprCprUprGprAprAprUprUprCprUprCprGprAprCprCp-3'
Molecular Weight: 27369.42
Extinction Coefficient: 805600
Binding Conditions / Buffer
Binding Buffer (20 mM NaxHyPO4 (pH 7.4),2 mM MgCl2,130 mM NaCl,5 mM KCl, 20 mM glucose,0.2 mM ??-mercaptoethanol)
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Homann and Goringer. "Combinatorial selection of high affinity RNA ligands to live African trypanosome." Nucleic Acid Research, 27(1999): 2006-2014.
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