Tobramycin (J6) Aptamer Details
ID# 7458
Structure
Chemistry
RNA
Target
Tobramycin
Category
Small Organic
Affinity (Kd)
2 nM
Length
109
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprGprAprGprAprAprUprUprCprCprGprAprCprCprAprGprAprAprGprCprUprUprAprGprUprAprUprAprGprCprGprAprGprGprUprUprUprAprGprCprUprAprCprAprCprUprCprGprUprGprCprUprGprAprUprCprGprUprUprUprGprGprUprAprCprGprGprGprAprCprCprUprGprCprGprUprGprUprAprGprCprCprCprAprUprAprUprGprUprGprCprGprUprCprUprAprCprAprUprGprGprAprUprCprCprUprCprAp-3'
Molecular Weight: 35049.02
Extinction Coefficient: 1078300
Binding Conditions / Buffer
Selection Buffer: 140 mM NaCl, 5 mM KCl, 1 mM CaCl2, 1mM MgCl2, and 20 mM Tris Acetate (pH 7.4)
Binding Temp: RT°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Wang, Yong, et al. "Specific binding of aminoglycoside antibiotics to RNA." Chemistry & Biology 2 (1995): 281-290
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