Thyroxine (ApT4-A’) Aptamer Details
ID# 7585
Structure
Chemistry
RNA
Target
Thyroxine
Category
Small Organic
Affinity (Kd)
50 µM
Length
44
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprUprGprGprAprGprGprGprGprGprAprCprGprUprGprCprUprGprCprAprUprCprCprGprCprAprGprUprGprCprGprUprCprUprUprGprGprGprUprUprGprUprGp-3'
Molecular Weight: 14313.6
Extinction Coefficient: 424000
Binding Conditions / Buffer
Loading buffer: 10mM Tris-HCl (pH 7.5), 500 mM NaCl, and 1 mM MgCl2
Binding Temp: 22°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Levesque et al. "In vitro selection and characterization of RNA aptamers binding thyroxine hormone." Biochemical Journal, 403(2007): 129-138.
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