Thrombin Aptamer Tb-L8 Aptamer Details
ID# 8062
Structure
Chemistry
DNA
Target
Thrombin
Category
Protein
Affinity (Kd)
33 nM
Length
44
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
dGpdGpdTpdGpdTpdGpdGpdGpdCpdCpdGpdApdCpdApdTpdGpdGpdGpdGpdGpdTpdTpdGpdGpdTpdGpdGpdTpdGpdTpdApdGpdGpdApdGpdCpdGpdCpdCpdApdTp
Molecular Weight: 12601.1
Extinction Coefficient: 389600
Binding Conditions / Buffer
50 mM HEPES, 100 mM NaCl, 1 mM MgCl2, 5 mM KCl, 1 mM,CaCl2, pH 7.4
Binding Temp: 20°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Qiao, N., Li, J., Wu, X., Diao, D., Zhao, J., Li, J., … Lou, X. (2019). Speeding up in vitro Discovery of Structure-Switching Aptamers via Magnetic Cross-Linking Precipitation. Analytical Chemistry. doi:10.1021/acs.analchem.9b00081
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