Aptagen

Thrombin Aptamer 2 Aptamer Details

ID# 8160
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Structure
Thrombin Aptamer 2 structure
Chemistry
DNA
Target
Thrombin
Category
Protein
Affinity (Kd)
0.027 nM
Length
147
Aptazyme
No

Sequence (3-char table code)

G A C U/T
dTpdTpdTpdTpdTpdTpdTpdTpdTpdTpdTpdTpdTpdTpdTpdTpdTpdTpdTpdTpdApdGpdTpdCpdCpdGpdTpdGpdGpdTpdApdGpdGpdGpdCpdApdGpdGpdTpdTpdGpdGpdGpdGpdTpdGpdApdCpdTp
Molecular Weight: 15448.88
Extinction Coefficient: 435546

Binding Conditions / Buffer

TE (20 mM Tris-HCl, 140 mM NaCl, 5 mM KCl, 5 mM MgCl2 and 1mM CaCl2, pH 7.4)
Binding Temp: 25°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

doi:10.1039/C7TB01276D
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