Thrombin (31mer) (D2-5) Aptamer Details
ID# 7787
Structure
Chemistry
DNA
Target
Thrombin
Category
Protein
Affinity (Kd)
N/A nM
Length
31
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dApdGpdCpdGpdAp dGpdTpdApdGpdGpdTpdTpdGpdGpdTpdGpdTpdGpdGpdTpdTpdGpdGpdGpdGpdCp dTpdCpdGpdCpdTp-3'
Molecular Weight: 9710.34
Extinction Coefficient: 295700
Binding Conditions / Buffer
Buffer for Clotting Inhibition assay: Tris-HCl pH 8.0, 5 mM KCl, 100 mM NaCl.
Binding Temp: 37°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
K Ikebukuro et al. Analysis of the evolution of the thrombin-inhibiting DNA aptamers using a genetic algorithm. Biotechnol Lett (2006) 28:1933ƒ??1937.
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