Thrombin (29mer) Aptamer Details
ID# 7699
Structure
Chemistry
DNA
Target
Thrombin
Category
Protein
Affinity (Kd)
0.5 nM
Length
29
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dApdGpdTpdCpdCpdGpdTpdGpdGpdTpdApdGpdGpdGpdCpdApdGpdGpdTpdTpdGpdGpdGpdGpdTpdGpdApdCpdTp-3'
Molecular Weight: 9085.94
Extinction Coefficient: 283600
Binding Conditions / Buffer
50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 1 mM MgCl2
Binding Temp: 37°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Tasset, D. (1997). Oligonucleotide inhibitors of human thrombin that bind distinct epitopes. J. Mol. Biol., 1997(272), 688-698.
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