Thrombin (15mer-29-mer dimer with 20T linker) Aptamer Details
ID# 7812
Structure
Chemistry
DNA
Target
Thrombin
Category
Protein
Affinity (Kd)
0.35 nM
Length
64
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdGpdTp dTpdGpdGp dTpdGpdTp dGpdGpdTp dTpdGpdGp dTpdTpdTpdTpdTp dTpdTpdTpdTpdTp dTpdTpdTpdTpdTp dTpdTpdTpdTpdTp dApdGpdTp dCpdCpdGp dTpdGpdGp dTpdApdGp dGpdGpdCp dApdGpdGp dTpdTpdGp dGpdGpdGp dTpdGpdAp dCpdTp-3'
Molecular Weight: 19958.03
Extinction Coefficient: 588600
Binding Conditions / Buffer
Binding Buffer: 50 mM Tris-HCl, 300 mM NaCl, 5 mM KCl, pH 8.0.
Binding Temp: 25°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
H. Hasegawa et al. Improvement of aptamer affinity by dimerization. Sensors 8(2008): 1090-1098.
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