Thrombin (15mer) Aptamer Details

ID# 7480

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Structure

Chemistry

DNA

Target

Thrombin

Sequence (3-char table code)

G A C U/T

5'-dGpdGpdTpdTpdGpdGpdTpdGpdTpdGpdGpdTpdTpdGpdGp-3'

Molecular Weight: 4726.13

Extinction Coefficient: 143300

Binding Conditions / Buffer

Selection Buffer: (20 mM Tris-acetate pH 7.4, 40mM NaCl, 5mM KCl, 1mM CaCl2, 1mM MgCl2)

Binding Temp: 37°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Bock, Louis, et al. "Selection of single-stranded DNA molecules that bind and inhibit human thrombin." Nature 355, (1992): 564-566. S Uehara et al. 30 Poly(dA)-Tailed Thrombin DNA Aptamer to Increase DNase-Resistance and Clotting Inhibitory Activity. Bull. Chem. Soc. Jpn. 81(2008): 1485ƒ??1491

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