Theophylline (mTCT8-4) Aptamer Details
ID# 7551
Structure
Chemistry
RNA
Target
Theophylline
Category
Small Organic
Affinity (Kd)
100 nM
Length
38
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rAprGprUprGprAprUprAprCprCprAprGprCprAprUprCprGprUprCprUprUprGprAprUprGprCprCprCprUprUprGprGprCprAprGprCprAprCprUp-3'
Molecular Weight: 12097.29
Extinction Coefficient: 364600
Binding Conditions / Buffer
Buffer A (100 mM Hepes (pH 7.3), 5 mM MgCI2, and 0.5 M NaCI)
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Jenison et al. "High-resolution molecular discrimination by RNA." Science, 263(1994): 1425-1429.
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