theophylline-induced molecular switch Aptamer Details
ID# 7762
Structure
Chemistry
RNA
Target
theophylline
Category
Small Organic
Affinity (Kd)
na nM
Length
76
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprGprCprGprAprCprCprCprUprGprAprUprGprAprGprCprCprUprUprAprUprAprCprCprAprGprCprCprGprAprAprAprGprGprCprCprCprUprUprGprGprCprAprGp rAprCprGprUprCprGprAprAprAprCprGprGprUprGprAprAprAprGprCprCprGprUprAprGprGprUprUprGprCprCprCp-3'
Molecular Weight: 24536.86
Extinction Coefficient: 736500
Binding Conditions / Buffer
Reaction buffer [50 mM Tris.HCl (pH 7.5) and 20 mM MgCl2] for 20 hr
Binding Temp: 23°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Garrett A. Soukup and Ronald R. Breaker. "Engineering precision RNA molecular switches" Proc. Natl. Acad. Sci. 96(1999):3584-3589.
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