TH14 Aptamer Details
ID# 9533
Structure
Chemistry
RNA
Target
BACE1-CT
Category
Protein
Affinity (Kd)
npBACE 360 and 280 nM
Length
90
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprGprAprUprAprGprGprAprUprCprCprAprCprAprUprCprUprAprCprGprUprAprUprUprAprCprGprCprAprAprCprGprCprCprGprGprGprCprCprAprCprUprAprCprGprCprGprAprAprUprGprGprCprAprAprGprCprCprCprGprUprCprGprAprCprAprAprGprCprUprUprCprGprUprCprAprAprGprUprCprUprGprCprAprGprUprGprAprAp-3'
Molecular Weight: 27692.9 g/mole
Extinction Coefficient: 856800
Binding Conditions / Buffer
100 mL binding buffer (PBS, 1 mM MgCl2 at pH 7.4)
Binding Temp: 25°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Rentmeister A, A Bill, T Wahle, J Walter and M Famulok. (2006). RNA aptamers selectively modulate protein recruitment to the cytoplasmic domain of b-secretase BACE1 in vitro. RNA 12:1650–1660. DOI: 10.1261/rna.126306
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