Tetracycline Aptamer Details
ID# 7587
Structure
Chemistry
DNA
Target
Tetracycline
Category
Small Organic
Affinity (Kd)
63.6 nM
Length
84
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dCpdGpdTpdApdCpdGpdGpdApdApdTpdTpdCpdGpdCpdTpdApdGpdCpdCpdCpdCpdCpdCpdGpdGpdCpdApdGpdGpdCpdCpdApdCpdGpdGpdCpdTpdTpdGpdGpdGpdTpdTpdGpdGpdTpdCpdCpdCpdApdCpdTpdGpdCpdGpdCpdGpdTpdGpdGpdApdTpdCpdCpdGpdApdGpdCpdTpdCpdCpdApdCpdGpdTpdGp-3'
Molecular Weight: 23367.05
Extinction Coefficient: 688400
Binding Conditions / Buffer
Binding Buffer: 100 mM NaCl, 20 mM Tris-HCl (pH 7.6), 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, 0.02% Tween-20
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Niazi JH, Lee SJ, Gu MB. (2008) Single-stranded DNA aptamers specific for antibiotics tetracyclines. Bioorg Med Chem. 16: 7245-7253.
Kim YJ, Kim YS, Niazi JH, Gu MB. (2010) Electrochemical aptasensor for tetracycline detection. Bioprocess Biosyst Eng. 33:31-37.
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