Tenascin-C (GBI-10) Aptamer Details
ID# 7477
Structure
Chemistry
DNA
Target
Tenascin-C (TNC)
Category
Protein
Affinity (Kd)
150 nM
Length
69
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdGpdCpdTpdGpdTpdTpdGpdTpdGpdApdGpdCpdCpdTpdCpdCpdTpdCpdCpdCpdApdGpdApdGpdGpdGpdApdApdGpdApdCpdTpdTpdTpdApdGpdGpdTpdTpdCpdGpdGpdTpdTpdCpdApdCpdGpdTpdCpdCpdCpdGpdCpdTpdTpdApdTpdTpdCpdTpdTpdApdCpdTpdCpdCpdCp -3'
Molecular Weight: 21127.69
Extinction Coefficient: 626300
Binding Conditions / Buffer
Binding Buffer (10 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM MgCl2, 1 mM CaCl2, 0.1% (wt/vol) BSA, 0.1 % (vol/vol) Triton X-100, 0.1% (vol/vol) Tween 20)
Binding Temp: 4°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Dion, Daniels, et al. "A tenascin-C aptamer identified by tumor cell SELEX: Systematic evolution of ligands by exponential enrichment." PNAS 100(2003): 15416-15421.
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