TAT RNA Aptamer TAT-X Aptamer Details

ID# 8412

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Structure

Chemistry

RNA

Target

Recombinant Trans-Activator of Transcription Aptamer binding Protein (RBP-mCherry-RBP)

Sequence (3-char table code)

G A C U/T

5'-rGprGprGprAprAprGprCprUprUprGprAprUprCprCprCprGprUprUprUprGprCprCprGprGprUprCprGprAprUprCprGprCprUprUprCprCprCprCprCprUprGprUprAprCprGprUprGprAprAprAprGprCprCprGprAprAprGprCprUprUprGprAprUprCprCprCprGprUprUprUprGprCprCprGprGprUprCprGprAprUprCprGprCprUprUprCprGprGprCprUprUprUprCprAprCprUprCprCprCprGprCprGprCprCprGprAprAprGprCprUprUprGprAprUprCprCprCprGprUprUprUprGprCprCprGprGprUprCprGprAprUprCprGprCprUprUprCprGprGprCprGprCprGprGprGprAprUprAprUprUprCprGprGprCprCprGprAprAprGprCprUprUprGprAprUprCprCprCprGprUprUprUprGprCprCprGprGprUprCprGprAprUprCprUprUprGprGprCprCprGprAprAprUprAprGprUprAprCprAprGprGp-3'

Molecular Weight: 67.32 kDa

Extinction Coefficient:

Binding Conditions / Buffer

50 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA, pH 7.4.

Binding Temp: 25°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Zou Z, Younas T, Dumsday G, Haritos VS, He L. Rapid production of multimeric RNA aptamers stabilized by a designed pseudo-circular structure in E. coli. Biotechnol J. 2023 Mar;18(3):e2200390. doi: 10.1002/biot.202200390. Epub 2022 Dec 5. PMID: 36427490.

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