Taq DNA polymerase (TQ21) Aptamer Details
ID# 7486
Structure
Chemistry
DNA
Target
Taq DNA polymerase
Category
Protein
Affinity (Kd)
36 pM
Length
78
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dTpdTpdCpdTpdCpdGpdGpdTpdTpdGpdGpdTpdCpdTpdCpdTpdGpdGpdCpdGpdGpdApdGpdCpdGpdApdTpdCpdApdTpdCpdTpdCpdApdGpdApdGpdCpdApdTpdTpdCpdTpdTpdApdGpdCpdGpdTpdTpdTpdTpdGpdTpdTpdCpdTpdTpdGpdTpdGpdTpdApdTpdGpdApdTpdTpdCpdGpdCpdTpdTpdTpdTpdCpdCpdCp-3'
Molecular Weight: 23951.56
Extinction Coefficient: 703200
Binding Conditions / Buffer
Binding Buffer (50 mM KCl, 2.5 mM MgCl2, 10 mM Tris-HCl (pH 8.3))
Binding Temp: RT°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Sumedha and Dang. "Oligonucleotide inhibitors of Taq DNA polymerase facilitate detection of low copy number targets by PCR." Journal of Molecular Biology, 264 (1996): 268-78.
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