TAFapt1-S Aptamer Details
ID# 8078
Structure
Chemistry
RNA
Target
AML1-MTG8
Category
Protein
Affinity (Kd)
4.47 nM
Length
37
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprGprUprUprUprUprCprGprGprGprAprUprAprCprGprCprGprGprAprGprGprGprUprGprGprGprCprAprAprUprAprAprCprCprCprCp-3'
Molecular Weight: 11413.3
Extinction Coefficient: 348600
Binding Conditions / Buffer
Binding buffer [20 mM sodium phosphate (pH 6.5), 2 mM magnesium acetate, 200 mM potassium acetate, 5% glycerol, 0.05% Triton X-100, 5 mM b-mercaptoethanol].
Binding buffer [20 mM Tris/HCl (pH 7.5) containing 50 mM KCl and 1 mM DTT].
Binding Temp: 25°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Fukunaga, Junichi et al. “A G-quadruplex-forming RNA aptamer binds to the MTG8 TAFH domain and dissociates the leukemic AML1-MTG8 fusion protein from DNA.” FEBS letters vol. 594,21 (2020): 3477-3489. doi:10.1002/1873-3468.13914
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