T20 Aptamer Details
ID# 8233
Structure
Chemistry
DNA
Target
Tetracycline
Category
Small Organic
Affinity (Kd)
63.6 nM
Length
76
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dCpdGpdTpdApdCpdGpdGpdApdApdTpdTpdCpdGpdCpdTpdApdGpdCpdCpdCpdCpdCpdCpdGpdGpdCpdApdGpdGpdCpdCpdApdCpdGpdGpdCpdTpdTpdGpdGpdGpdTpdTpdGpdGpdTpdCpdCpdCpdApdCpdTpdGpdCpdGpdCpdGpdTpdGpdGpdApdTpdCpdCpdGpdApdGpdCpdTpdCpdCpdApdCpdGpdTpdGp-3'
Molecular Weight:
Extinction Coefficient:
Binding Conditions / Buffer
Briefly, the target chemical-coated magnetic
beads were washed several times with binding buffer containing
NaCl (100 mM), Tris–HCl (20 mM, pH 7.6), MgCl2(2 mM), KCl
(5 mM), CaCl2(1 mM), and Tween 20 (0.02%) before each round of
SELEX process.
Binding Temp: 25°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Niazi, J. H., Lee, S. J., & Gu, M. B. (2008). Single-stranded DNA aptamers specific for antibiotics tetracyclines. Bioorganic & medicinal chemistry, 16(15), 7245–7253. https://doi.org/10.1016/j.bmc.2008.06.033
https://pubmed.ncbi.nlm.nih.gov/18617415/
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