T2 Aptamer Details
ID# 8139
Structure
Chemistry
DNA
Target
Plasmodium vivax lactate dehydrogenase (PvLDH)
Category
Protein
Affinity (Kd)
670 nM
Length
79
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5’-dCpdApdCpdTpdCpdApdCpdGpdTpdCpdApdGpdTpdGpdApdCpdApdTpdGpdCpdApdTpdGpdCpdCpdGpdApdTpdGpdApdCpdTpdApdGpdTpdCpdGpdTpdCpdApdCpdTpdApdGpdTpdGpdCpdApdCpdGpdTpdApdApdCpdGpdTpdGpdCpdTpdApdGpdTpdCpdApdGpdApdApdApdTpdTpdTpdCpdGpdCpdApdCpdCpdApdCp-3’
Molecular Weight: 24238.7
Extinction Coefficient: 755500
Binding Conditions / Buffer
25 mM Tris·HCl at pH 7.5 containing 0.1 M NaCl and 20 mM imidazole
Binding Temp: 22°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Cheung YW, Röthlisberger P, Mechaly AE, et al. Evolution of abiotic cubane chemistries in a nucleic acid aptamer allows selective recognition of a malaria biomarker. Proc Natl Acad Sci U S A. 2020;117(29):16790-16798. doi:10.1073/pnas.2003267117
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