SW1A Aptamer Details
ID# 8183
Structure
Chemistry
DNA
Target
SMMC-7721 cells (liver cancer)
Category
Protein
Affinity (Kd)
123.62 ± 17.53 nM
Length
35
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dApdGpdGpdCpdCpdCpdGpdCpdCpdGpdGpdGpdTpdGpdGpdGpdTpdGpdGpdGpdGpdGpdGpdGpdTpdTpdTpdGpdCpdGpdGpdGpdCpdCpdTp-3'
Molecular Weight:
Extinction Coefficient:
Binding Conditions / Buffer
Washing Buffer: 0.01 M DPBS, 5 mmol/L MgCl2, and 4.5 mg/mL glucose
Blocking Buffer: Washing Buffer with additional 0.1 mg/mL yeast tRNA, 1 mg/mL BSA, and 0.5 mg/mL salmon DNA (Solarbio)
Binding Buffer: Washing Buffer with additional 1 mg/L bovine serum albumin (BSA, Dingguo) and 0.1 mg/mL yeast tRNA (Sigma)
Binding Temp: 37°C
Refolding Protocol
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Li, L., Wan, J., Wen, X., Guo, Q., Jiang, H., Wang, J., … Wang, K. (2021). Identification of a New DNA Aptamer by Tissue-SELEX for Cancer Recognition and Imaging. Analytical Chemistry, 93(19), 7369–7377. doi:10.1021/acs.analchem.1c01445
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