Sulforhodamine B (Clone 73 – min) Aptamer Details
ID# 7549
Structure
Chemistry
DNA
Target
Sulforhodamine B
Category
Small Organic
Affinity (Kd)
190 nM
Length
29
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dCpdCpdGpdGpdCpdCpdApdApdGpdGpdGpdTpdGpdGpdGpdApdGpdGpdGpdApdGpdGpdGpdGpdGpdCpdCpdGpdGp -3'
Molecular Weight: 9155.94
Extinction Coefficient: 284400
Binding Conditions / Buffer
Selection Buffer: 0.1 M KCl, 5 mM MgCl2, 10 mM Na-HEPES (pH 7.4).
Binding Temp: RT°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Wilson C, Szostak J. (1998) Isolation of a fluorophore-specific DNA aptamer with redox activity. Chem Biol. 5(11):609-617.
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