Stx 2 B Aptamer (B2) Aptamer Details

ID# 8776

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Structure

Chemistry

DNA

Target

Stx 2 B (Shiga toxin type 2 B Subunit)

Sequence (3-char table code)

G A C U/T

5'-dGpdGpdCpdApdApdCpdGpdGpdTpdCpdTpdTpdTpdGpdCpdTpdApdTpdTpdTpdApdCpdGpdGpdCpdApdApdCpdApdCpdCpdGpdCp-3'

Molecular Weight: 10,890

Extinction Coefficient:

Binding Conditions / Buffer

Binding buffer solution, 4 μM target, and 5 μM aptamer; incubated (2 hours)

Binding Temp: 37°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Duan M, Ren K, Chen X, Chang Y, Lv Z, Wang Z, Wu S, & Duan N. (2024). "Discovery and design of an aptamer that inhibits Shiga toxin type 2 activity by blocking Stx 2 B subunit-Gb3 interaction." International Journal of Biological Macromolecules, (277)134365. https://doi.org/10.1016/j.ijbiomac.2024.134365

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