Streptavidin (31) Aptamer Details
ID# 7471
Structure
Chemistry
DNA
Target
Streptavidin
Category
Protein
Affinity (Kd)
56.7 nM
Length
96
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dApdTpdApdCpdCpdApdGpdCpdTpdTpdApdTpdTpdCpdApdApdTpdTpdCpdTpdApdTpdApdCpdTpdCpdCpdApdCpdTpdTpdTpdGpdCpdTpdApdTpdTpdTpdCpdTpdCpdGpdGpdTpdTpdCpdCpdTpdTpdCpdApdCpdGpdCpdGpdCpdCpdGpdApdTpdCpdGpdCpdApdGpdGpdCpdTpdGpdApdTpdGpdApdApdTpdTpdGpdApdGpdApdTpdApdGpdTpdApdApdGpdTpdGpdCpdApdApdTpdCpdTp-3'
Molecular Weight: 29413.16
Extinction Coefficient: 903200
Binding Conditions / Buffer
Binding buffer: 100 mM NaCl, 20 mM, Tris-HCl (pH 7.6), 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, 0.02% Tween 20
Binding Temp: 21°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Stoltenburg et al. "FluMag-SELEX as an advantageous method for DNA aptamer selection." Analytical and Bioanalytical Chemistry, 383 (2005): 83-91.
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