StrepApt5 Aptamer Details
ID# 7906
Structure
Chemistry
DNA
Target
Streptavidin
Category
Protein
Affinity (Kd)
35 nM
Length
26
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
dGpdGpdGpdApdApdCpdGpdCpdApdCpdCpdGpdApdTpdCpdGpdCpdApdGpdGpdTpdTpdTpdCpdCpdCp
Molecular Weight: 7957.19
Extinction Coefficient: 242700
Binding Conditions / Buffer
NaCl (100 mM), MgCl2 (2 mM), KCl (5 mM), CaCl2 (1 mM), Tris-HCl (pH 7.6, 20 mM)
Binding Temp: heated to 95C for 8 minutes, cool on ice for 10 minutes and left at room temperature for at least 10 minutes.
Refolding Protocol
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Ruigrok, Vincent J. B., et al. "Kinetic and Stoichiometric Characterisation of Streptavidin-Binding Aptamers." ChemBioChem 13 (2012): 829-836. Print.
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