STR1 Aptamer Details

ID# 8231

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Structure

Chemistry

DNA

Target

Streptomycin

Sequence (3-char table code)

G A C U/T

5'-dTpdApdGpdGpdGpdApdApdTpdTpdCpdGpdTpdCpdGpdApdCpdGpdGpdApdTpdCpdCpdGpdGpdGpdGpdTpdCpdTpdGpdGpdTpdGpdTpdTpdCpdTpdGpdCpdTpdTpdTpdGpdTpdTpdCpdTpdGpdTpdCpdGpdGpdGpdTpdCpdGpdTpdCpdTpdGpdCpdApdGpdGpdTpdCpdGpdApdCpdGpdCpdApdTpdGpdCpdGpdCpdCpdGp-3'

Molecular Weight:

Extinction Coefficient:

Binding Conditions / Buffer

Binding buffer used to wash samples was as follows: 20 mM Tris–HCl containing 100 mM NaCl, 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, and 0.02% Tween 20, pH 7.6.

Binding Temp: 20°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Zhou N, Wang J, Zhang J, Li C, Tian Y, Wang J. Selection and identification of streptomycin-specific single-stranded DNA aptamers and the application in the detection of streptomycin in honey. Talanta. 2013 Apr 15;108:109-16. doi: 10.1016/j.talanta.2013.01.064. Epub 2013 Feb 28. PMID: 23601877.

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