Aptagen

Staphylococcus aureus (SA23) Aptamer Details

ID# 7562
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Structure
Staphylococcus aureus (SA23) structure
Chemistry
DNA
Target
Staphylococcus aureus
Category
Cells
Affinity (Kd)
61.5 nM
Length
88
Aptazyme
No

Sequence (3-char table code)

G A C U/T
5'-dGpdCpdApdApdTpdGpdGpdTpdApdCpdGpdGpdTpdApdCpdTpdTpdCpdCpdGpdGpdGpdCpdTpdGpdGpdCpdCpdApdGpdApdTpdCpdApdGpdApdCpdCpdCpdCpdGpdGpdApdTpdGpdApdTpdCpdApdTpdCpdCpdTpdTpdGpdTpdGpdApdGpdApdApdCpdCpdApdCpdApdApdApdApdGpdTpdGpdCpdApdCpdGpdCpdTpdApdCpdTpdTpdTpdGpdCpdTpdApdAp-3'
Molecular Weight: 27104.61
Extinction Coefficient: 842400

Binding Conditions / Buffer

Screening buffer: 0.1 mg/ml salmon sperm DNA (0.1 mg/ml tRNA, 1%BSA, 1 PBS, 0.05% (v/v) Tween-20
Binding Temp: 37°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Cao et al. "Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureus." Nucleic Acids Research, 37(2009): 1-8
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