Aptagen

Staphylococcus aureus Protein A (SpA) (fmA12) Aptamer Details

ID# 10011
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Structure
Staphylococcus aureus Protein A (SpA) (fmA12) structure
Chemistry
RNA
Target
Staphylococcus aureus Protein A (SpA)
Category
Protein
Affinity (Kd)
67.1 ± 6.1 nM
Length
40
Aptazyme
No

Sequence (3-char table code)

G A C U/T
5'-mUpfGpmUpmApmApmUpmUpmCpmUpfGpmCpmCpmApmUpmUpmCpmUpmUpmUpmUpmUpfGpfGpfGpfGpmCpfGpfGpmApmApmUpmApmCpmApfGpfGpmApmUpfGpmUp-3'
Molecular Weight: 12152.6 g/mole
Extinction Coefficient: 363100

Binding Conditions / Buffer

20 mM HEPES, pH 7.4, 150 mM sodium chloride, 5 mM magnesium chloride, 1 mM calcium chloride, 1 mg/mL BSA, 0.1 mg/mL yeast tRNA
Binding Temp: Room temperature°C

Refolding Protocol

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Friedman AD, Kim D, Liu R. Highly stable aptamers selected from a 2'-fully modified fGmH RNA library for targeting biomaterials. Biomaterials. 2015 Jan;36:110-23. doi: 10.1016/j.biomaterials.2014.08.046.
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